Evaluation of wound healing effects between Salvadora a persica ointment and Solcoseryl jelly in animal model

In this research study very first time a herbal ointment contain 10% Salvadora persica extract was compared with Solcosseryl jelly 10% and blank Vaseline to evaluate wound healing effects using excision wound healing model in animals. Three groups of rats [n-6] were experimentally wounded on the back of their neck. Group I was dressed with Vaseline containing 10% test drug, Group II was treated with thin layer of Solcoseryl jelly 10% as reference drug while Group III was dressed with thin layer of blank Vaseline as control group. The effect of vehicle on rate of wound healing were assessed and in all cases there were progressive decreased in wound area with time but wound dress with Vaseline containing S. persica extract and wound treated with Solcosseryl jelly significantly healed earlier than those treated with Vaseline. It is concluded that S. persica extract significantly enhance the acceleration rate of wound enclosure in rats

Pharmacological rational of dry ripe fruit of Aegle marmelos L. as an anti-nociceptive agent in different painful conditions

The aim of study is to investigate central and peripheral analgesic effects of methanolic extract of dry ripe fruit of Aegle marmelos Linn. Corea [Am. Cr] by two methods, tail flick test and acetic acid induced writhing test at 100, 250 and 500mg/kg doses in animal models. Analgesic activity against tail flick test revealed that Am. Cr induced significant increase in latency period in dose dependent manner i.e. 65.38% at 100mg/kg, 395.37% at 250mg/kg [p<0.01] and 459.25% at 500mg/kg [p<0.01] body weight at 1hr after drug delivery while at 2hr effect decreased i.e. 61.53% at 100mg/kg, 161.11% [p<0.01] at 250mg/kg and 165.74% [p<0.01] at 500mg/kg but interestingly again there is an elongation in latency period at 3hr i.e. 106.15% at 100mg/kg dose, 251.85% [p<0.01] at 250mg/kg and 293.51% [p<0.05] at 500mg/kg respectively. The standard drug Diclofenac sodium at the dose of 5mg/kg continuously increased the latency period but less significantly as compared to the test substance i.e. 79.43%, 113.08% and 222.42% [p<0.05] respectively. Acetic acid induced writhing test produced highest significant activity at the dose of 100mg/kg i.e. 89.83% [p<0.01] as compared to Diclofenic sodium [standard drug] at a dose of 5mg/kg body weight i.e 63.63% [p<0.01]. It is concluded that dry ripe fruit of A. marmelos possesses significant dual analgesic activities i.e. central and peripheral

Evaluation of analgesic activity of P. domestica L.

This research study was conducted to investigate acute oral toxicity and analgesic activity of ethanol extract of P. domestica fruit by using tail flick analgesiometer at 300 and 500mg/kg doses in animal models. Acute oral toxicity results showed that crude extract is safe up to the dose of 5g/kg body weight of animals. The analgesic activity revealed that P. domestica extract at 500mg/kg dose possesses highest significant and prolonged analgesic activity in dose dependent manner as compared to standard and control groups. Aspirin 300mg/kg body weight was used as standard drug. Phytochemical analysis was also carried out which showed the presence of certain phytochemicals constituents in test drug that are responsible for analgesic activity. Therefore the results are justified

Pharmacological profile of salvadora persica

This work was conducted to investigate the various pharmacological activities of Salvadora. persica family Salvadoracea and that includes anti inflammatory, analgesic, CNS, bleeding and clotting time activity by oral administration at the dose of 300 and 500mg/kg of body weight in animal models. Acute oral toxicity results showed that crude extract of S. persica is safe up to the dose of 5g/kg body weight of animals. Carraganeen induced hind paw edema method for anti inflammatory activity, tail immersion test method for analgesic activity, Rota rod and grip strength test for CNS activity were carried out in animal models. The analgesic activity was compared with aspirin, 300mg/kg body weight, anti inflammatory activity was compared with indomethacine, lOmg/kg body weight, Transamin 250mg/kg and Vitamin K lOmg were used for bleeding and clotting time activity respectively while diazepam 5mg/kg were used as standard for behavior and CNS activities. In all activities S. persica showed prolonged and dose dependent effects. Phytochemical analysis was also carried out which showed the presence of certain phytoconstituents which possesses these properties. Therefore the results justified the traditional use of the plant

Efficacy of 0.3% topical ciprofloxacin and tobramycin ophthalmic solutions in the treatment of experimental Pseudomonas aeruginosa keratitis in rabbits

To compare the efficacy of topical ciprofloxacin 0.3% with tobramycin 0.3% ophthalmic solution for treating the Pseudomonas aeruginosa keratitis in the rabbits. P. aeruginosa was isolated from infected human eye of patients attending Akhtar Eye Hospital, Karachi. Rabbits were obtained from the animal house of Pakistan Council of Scientific and Industrial Research [PCSIR]. Experimental bacterial keratitis was induced in rabbits by corneal intrastromal injection. Infection proceeded for 24 hours; all eyes developed corneal ulcer without pretreatment with immunosuppressive agents. Topical ciprofloxacin 0.3% and tobramycin 0.3% ophthalmic solutions were applied in the eyes of test group rabbits and normal saline solutions 0.9% was applied in the eyes of control group rabbits for 72 hours respectively. Corneas were then removed and corneal homogenates were plated with serial dilutions for quantitative bacteriology. Culture results from 20 corneas of the test group rabbits and 6 of the control group were studied. In the test group that was treated with ciprofloxacin, 10 of 10 corneas were sterile while 5 of 10 corneas in the test group II that was treated with tobramycin were sterile after 72 hours of treatment. In control group 6 of 6 corneas were still positive. It is found that ciprofloxacin 0.3% is superior to tobramycin 0.3% solution in treating experimental P. aeruginosa keratitis in rabbit's model. It is concluded that ciprofloxacin 0.3% solution was superior for P. aeruginosa compared to tobramycin 0.3% solution in treating experimental keratitis in rabbit's model

Optimization of cultural conditions of penicillium griseofuhvum for the production of penicillin griseofulvin

The present study is mainly concerned with the production of Penicillin griseofulvum by Penicillium griseofulvum NRRL 2300 in Shake Flask Technique. The production of antibiatic was carried out in cotton wool plugged 250 ml flasks. 24 hours old, vegetative inoculum was developed in conical. flasks, containing 100 ml of basal medium. Fermentation, for antibiotic synthesis was carried out for 312 hours at 2S degree C. Effects of different nitrogen and carbon sources for the formation of antibiotic were evaluated. Maximum production of antibiotic was observed in the presence of corn steep liquor, which was used as a source of nitrogen. Maximum production of penicillin griseofulvin was also observed in the presence of sucrose as a source of carbon; whereas it was decreased in the presence of other carbon sources like lactose and glucose. Other conditions such as temperature and pH were also optimized for the production of griseofulvin